xklp2 targeting protein (interpro:ipr009675)  (InterPro Inc)

Journal: Oncology Letters

Article Title: Reversal of the tamoxifen‑resistant breast cancer malignant phenotype by proliferation inhibition with bromosulfonamidine amino‑podophyllotoxin

doi: 10.3892/ol.2024.14506

Figure Lengend Snippet: Quantitative PCR primers.

Article Snippet: Mouse anti-human Caspase-9 antibodies (cat. no. 9508S; 1:1,000) were purchased from Cell Signaling Technology, Inc., rabbit anti-human Bcl-2 (cat. no. BA0412; 1:1,000) and cyclin B1 (CCNB1; cat. no. BA0766; 1:1,000) antibodies were purchased from Wuhan Boster Biological Technology, Ltd., rabbit anti-human polo like kinase (PLK)-1 antibodies (cat. no. 10305-1-AP; 1:1,000) and targeting protein for Xklp2 (TPX2) antibodies (cat. no. 11741-1-AP; 1:2,000) were purchased from Proteintech Group, Inc., and the rabbit anti-human PLK-4 antibodies (cat. no. A9863; 1:1,000) were purchased from ABclonal Biotech Co., Ltd.

Techniques: Real-time Polymerase Chain Reaction, Sequencing

Journal: Oncology Letters

Article Title: Reversal of the tamoxifen‑resistant breast cancer malignant phenotype by proliferation inhibition with bromosulfonamidine amino‑podophyllotoxin

doi: 10.3892/ol.2024.14506

Figure Lengend Snippet: Effect of BSAPPT on apoptosis and cycle-related gene or protein expression in MCF-7, MCF7/TAMR and other cancer cells. (A) mRNA expression levels of genes linked to the cell cycle and apoptosis were measured by qPCR both before and after MCF-7 and MCF7/TAMR cells were treated with 10 µg/ml BSAPPT. (B) Western blotting detection of apoptotic and cycle-related protein expression variations in MCF-7 and MCF7/TAMR cells before and after using 10 µg/ml BSAPPT. Results of qPCR analysis that assessed differences in the level of expression of genes linked to the cell cycle and apoptosis before and after (C) A549 and (D) MDA-MB-231 cells were treated with 10 µg/ml BSAPPT. *P<0.05; **P<0.01; ***P<0.001. BSAPPT, bromosulfonamidine amino-podophyllotoxin; qPCR, quantitative PCR; Bcl-2, B-cell lymphoma 2; Caspase, cysteine aspartic acid-specific protease; PLK, polo like kinase; CCNB1, cyclin B1; TPX2, targeting protein for Xklp2; Bax, Bcl-2 associated X; Cyt-C, cytochrome c; Apaf-1, apoptotic protease activating factor 1.

Article Snippet: Mouse anti-human Caspase-9 antibodies (cat. no. 9508S; 1:1,000) were purchased from Cell Signaling Technology, Inc., rabbit anti-human Bcl-2 (cat. no. BA0412; 1:1,000) and cyclin B1 (CCNB1; cat. no. BA0766; 1:1,000) antibodies were purchased from Wuhan Boster Biological Technology, Ltd., rabbit anti-human polo like kinase (PLK)-1 antibodies (cat. no. 10305-1-AP; 1:1,000) and targeting protein for Xklp2 (TPX2) antibodies (cat. no. 11741-1-AP; 1:2,000) were purchased from Proteintech Group, Inc., and the rabbit anti-human PLK-4 antibodies (cat. no. A9863; 1:1,000) were purchased from ABclonal Biotech Co., Ltd.

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction